Search results for "Genetic transformation"
showing 7 items of 7 documents
Citrus biotechnology
2020
Agricultural crops that can better withstand the changing climatic and pathogen landscape have been produced through natural selection throughout the millennia and, in recent years, through the process of human-assisted plant breeding and selection. However, a lack of genetic diversity in many commercially cultivated crops (due to monoculture) has made them more vulnerable to biotic and abiotic stresses (Esquinas-Alcazar, 2005; Keneni et al., 2012). Citrus, belonging to the Rutaceae family, is one of the most important commercial woody fruit crops in the tropical and subtropical areas of the world. The origin of citrus is traced back to parts of tropical and subtropical Southeast Asia (Wu e…
Transformation of Aspergillus parasiticus using autonomously replicating plasmids from Aspergillus nidulans.
1994
A genetic transformation system for the aflatoxin-producing fungus Aspergillus parasiticus using two autonomously replicating plasmids from A. nidulans (ARp1 and pDHG25) is reported. Transformation frequencies using the plasmid pDHG25 were from 5 x 10(2) to 2.5 x 10(4) transformants per 10(6) viable protoplasts and microgram DNA. The stability of the plasmids in the transformants was also studied. This transformation system offers a new opportunity to clone genes related to aflatoxin production using appropriate aflatoxin-defective mutants.
Non-target and biological diversity risk assessment
2008
This chapter discusses the following procedures for risk assessment in Bt cotton using the non-target risk assessment model developed by scientists of the GMO ERA Project ("International Project on GMO Environmental Risk Assessment Methodologies", which is a continuation of the GMO Guidelines Project, which was launched by scientists of the International Organization for Biological Control Global Working Group on "Transgenic Organisms in Integrated Pest Management and Biological Control"): (1) identify relevant functional groups of biological diversity associated with adverse effects, (2) list and prioritize species or ecological processes, (3) identify potential exposure pathways and adver…
Dissecting the factors controlling seed development in the model legume Medicago truncatula
2012
Legumes are not only indispensible for sustainable agriculture but are also a rich source of protein in food and feed for humans and animals, respectively. However, major proteins stored in legume seeds are poor in sulfur-containing amino acids, and may be accompanied by anti-nutritional factors causing low protein digestibility problems. In this regard, Medicago truncatula serves as a model legume to study legume seed development especially the phase of seed storage protein accumulation. As developing legume seeds are complex structures, a thorough knowledge of the morphogenesis of the seed and the characterization of regulatory mechanisms underlying the embryo development and seed filling…
Grass Pea
2011
Grass pea (Lathyrus. sativus L.) is an annual legume crop with high protein content and remarkable resistance to extreme environmental conditions, including fl ooding, drought, salinity and low soil fertility, and a signifi cant degree of resistance to biotic stress agents. It is rightly considered one of the most promising sources of calories and protein for the vast and expanding populations of drought-prone and marginal areas of Asia and Africa and as an interesting alternative for cropping systems diversifi cation in marginal lands in Europe, Australia and America. It is a dual purpose crop with great agronomic potential as a grain and forage legume. Nevertheless, as a result of the lit…
Holm Oak Somatic Embryogenesis: Current Status and Future Perspectives
2019
Quercus ilex (holm oak) is one of the most representative trees in the Mediterranean basin, but now the sustainability of its ecosystems is at serious risk due to the lack of natural regeneration and to the presence of a severe disease called oak decline that has caused the death of thousands of trees. The application of biotechnological tools, such as somatic embryogenesis, allows programs of genetic improvement of the species to be speeded up and helps in the conservation of its ecosystems. Somatic embryogenesis is currently considered one of the main biotechnological techniques that has demonstrated significant benefits when has applied to forest tree species, providing significant advan…
Exclusion of PAX9 and MSX1 mutation in six families affected by tooth agenesis. A genetic study and literature review
2013
Objectives: In the present study, it is described the phenotypical analysis and the mutational screening, for genes PAX9 and MSX1, of six families affected by severe forms of tooth agenesis associated with other dental anomalies and systemic entities. Study Design: Six families affected by severe tooth agenesis associated with other dental anomalies and systemic entities were included. Oral exploration, radiological examination, medical antecedents consideration and mutational screening for PAX9 and MSX1 were carried out. Results: No mutations were discovered despite the fact that numerous teeth were missing. An important phenotypical variability was observed within the probands, not being …